The ratio of diameter of objective lens to focal length is:
a. Numerical aperture
b. Resolution
c. Magnification
d. None of these
An example for positive eye piece:
a. Huygenian eyepiece
b. Ramsden eyepiece
c. Both a and b
d. None of these

________________ microscopy is used to determine dry mass of living cells and their nuclei
a. Darkfield microscopy
b. Laser microscopy
c. Interference microscopy
d. Flourescence microscopy

________________ types of specimens have to be used in electron microscopy
a. Hydrated specimen
b. Dehydrated specimen
c. Thick specimen
d. Stained specimen

Which among the following contain sterol in cytoplasmic membrane
a. Spirochetes
b. Mycoplasma
c. Clamydia
d. Clostridia

_________________ are also known as PPLO
a. Rickettsiae
b. Mycoplasma
c. Mycobacteria
d. Clamydia

________________ produces packet of 8 organisms or multiple of 8.
a. Neisseria
b. Sarcina
c. Veillonella
d. All the above

Normal fir tree appearence in gelatin stab medium is produced by
a. Bacillus anthracis
b. Clostridium
c. Erysipelothrix
d. Salmonella

Primary stain in gram staining is
a. Crystal violet
b. Methylene blue
c. Safranin
d. Carbol fuchsin

In Hucker staining method, gram positive cell appear ________________ in colour
a. Blue
b. Pink
c. Violet
d. Dark red

Which among the following is wrong about negative staining of bacteria?
a. Used to study cell shape
b. Can be done with eosin or nigrosin
c. Stain does not penetrate the cell
d. Heat fixation is required

Which among the following is acid fast positive?
a. Mycoplasma
b. Nocardia
c. Neisseria
d. Fusobacterium

Traunt staining fluorescence method is used for
a. Endoscope
b. Acid fast bacteria
c. Flagella
d. Gram positive bacteria

Popping test is done for the staining of
a. Exospore
b. Endospore
c. Pili
d. Flagella

Which among the following is used for endospore staining
a. Schaeffer-Fulton staining
b. Donner staining method
c. Burke staining method
d. Both a and b

Which among the following is used for flagellar staining?
a. Gray staining method
b. Leyson staining method
c. Dorner staining method
d. Both a and b

______________ is used in Ames test
a. Salmonella typhimurium
b. Shigella dysentriae
c. Staphylococcus aureus
d. Sreptococcus pneumoniae

Muller Hinton agar is used in
a. Ames test
b. Replica plating method
c. Antibiotic sensitivity test
d. Penicillin enrichment of mutants

Calcium chloride treated bacterial cells are competent for
a. Transduction
b. Transformation
c. Plasmid replication
d. Conjugation

Indicator in urea broth medium is _____________________
a. Phenol red
b. Bromocresol purple
c. Methyl red
d. Bromothymol blue

IMViC reaction for Yersinia is
a. + + – –
b. – + – +
c. – – + +
d. – + – –

Indole production can be detected by _____________________
a. Barritt’s reagent
b. Kovac’s reagent
c. Collin’s reagent
d. Baeyer’s reagent

Which among the following shows mixed acid fermentation of glucose
a. Shigella
b. Klebsiella
c. Enterobacter
d. Serratia

Which among the following is used as cryoprotectants in the storage of bacterial culture at -700C?
a. 10% glycerol
b. 10% DMSO
c. 15% glycerol
d. 15% DMSO

Voges proskeur test is based on the ability of organism to produce ________________ in glucose phosphate broth
a. Urea
b. Pyruvic acid
c. Acetoin
d. Phenylene diamine

ANSWERS: 1. a 2. b 3. c 4. b 5. b 6. b 7. b 8. b 9. a 10. c 11. d 12. b 13. b 14. b 15. d 16. d 17. a 18. c 19. b 20. a 21. d 22. b 23. a 24. a 25. c